Factsheet – Pilot study to evaluate potential use of fatty acids as condition index of freshwater mussels (Unionidae) in response to upstream environmental effects

A research project funded by the Indiana Water Resources Research Center through the U.S. Geological Survey’s 104B annual base grants (section 104 of the Water Resources Research Act of 1984, as amended).

Start Date: 2021-09-01

End Date: 2022-08-31

Total Federal Funds: $16,750

Total Non-Federal Funds: $33,900

Freshwater mussels (Unionidae) are a diverse and ecologically important group of organisms that are globally distributed and widespread across North America. In Indiana and across their range, unionid mussels have experienced drastic losses in diversity and abundance, and many extant species are considered threatened or endangered. Efforts to conserve mussel populations are currently underway by a number of agencies and institutions, but these efforts may be hindered by knowledge gaps related to basic life history, ecology, and mechanisms related to mussel population health. While researchers have utilized quantitative modeling as a tool to better understand mussel distributions, few mechanistic approaches have been developed to directly measure individual mussel condition, population health, and more recent species’ responses to environmental stressors. Profiles of fatty acids, a critical macronutrient, are often directly indicative of a consumer’s diet and the habitats they occupy. This project explored the use of fatty acid profiles to assess mussel health and performance.

Research Objectives

Develop a proof of concept of how fatty acid profiles differ among freshwater mussels originating from habitats with contrasting degrees of environmental impairment.

We targeted a relatively abundant and ubiquitous mussel species, Wabash Pigtoe (Fusconaia flava), and collected individuals from six locations (across three rivers: Tippecanoe River, White River and Mississinewa River) in the Ohio River drainage. Locations were paired within a single river, with one site being upstream and one site downstream of a dam. After collecting mussels, we measured their fatty acid profiles using a gas chromatography-mass spectrometer.

Wabash Pigtoe mussels
Wabash Pigtoe mussels collected in the White River (Indiana) for subsequent fatty acid analysis. Photo credit: Brant Fisher and Ben Szczygiel

Researcher Profile

Dr. Tomas Hook

Principal Investigator Dr. Tomas Höök is a Professor in the Department of Forestry & Natural Resources and at Purdue University and Director of the Illinois-Indiana Sea Grant.

Major Conclusions & Significance

The major conclusions of this study are that in our model species, Wabash Pigtoe (Fusconaia flava), fatty acid profiles are variable across sites in the Ohio River drainage. We believe this largely reflects differences in the types and amounts of food which mussels are able to filter and consume at these different locations. In addition, differences in environmental conditions may influence fatty acid expression across these locations.

These pilot study findings are encouraging in that they suggest that fatty acids may be a useful approach to assess mussel condition. Next steps should include exploration of how different food types and environmental conditions translate to specific fatty acid profiles and if similar responses are evident across different mussel species.

What Does This Mean For Indiana?

These results are particularly informative for managers and researchers involved with the conservation and study of freshwater mussels. The reasons for mussel declines are likely numerous and often the mechanisms leading to a particular decline are unknown. In addition, the current health and condition of mussels can be difficult to assess, limiting the ability to detect likely declines ahead of time. This study demonstrates the high variability of fatty acids in mussels and may suggest that fatty acids could be used as an index of mussel condition and a potential harbinger of future declines.

Training The Next Generation

One of the missions of the Indiana Water Resources Research Center, and all Water Centers, is to train the next generation of water scientists. This project successfully funded research for one Masters student within Dr. Höök’s lab.

 

Contact Laura Esman, Managing Director, to request a printed copy of this factsheet.

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