Factsheet – Cyanophages technology: A novel and eco-friendly method to control cyanobacteria in Indiana lakes

A research project funded by the Indiana Water Resources Research Center through the U.S. Geological Survey’s 104B annual base grants (section 104 of the Water Resources Research Act of 1984, as amended).

Start Date: 2022-09-01

End Date: 2023-08-31

Total Federal Funds: $25,000

Total Non-Federal Funds: $28,471

Excess nutrients in the freshwater environment stimulate harmful algal blooms (HABs), which rapidly increase and accumulate in lakes and rivers when the optimum environmental conditions are met. The overabundance of HABs in freshwater increases the risk to human and animal health, reduces water transparency, creates oxygen-deprived aquatic zones, leads to death of dwelling plants and fishes, and ultimately decreases the recreational use of water. HABs are especially important in the water body if the water is used as a municipal drinking water reservoir. Cyanobacteria are a type of blue-green algae that form harmful (toxic, food web changing, hypoxia and anoxia generating) blooms in water ecosystems. Innovative approaches are needed to prevent cyanobacteria occurrence, accumulation, and transport in aquatic biota. Cyanophage could be used to control the overgrowth of targeted cyanobacteria in Indiana lakes. The engineering objective of this study was to explore cyanophage-based viral control systems for moderating and controlling cyanobacteria in the lake environment. Cyanophage therapy is a novel and environmentally friendly approach that can be applied as needed.

Research Objectives

1. To grow pure culture cyanobacteria in the laboratory to define the growth and environmental conditions affecting cyanobacteria and toxin production. The growth conditions for cyanobacteria need to be better understood.

2. Once we establish optimal cyanobacteria growth conditions, define how to introduce and monitor the actions of cyanophage relative to the cyanobacteria population.

3. To investigate a type of dominant cyanobacteria in the lake environment in Indiana and plan to control them using appropriate cyanophage. 

Bacteriophage
Figure 1. Schematic representation of bacteriophage prototype.

Researcher Profile

Dr. Halis Simsek

Principal Investigator Dr. Halis Simsek is an Assistant Professor of Agricultural and Biological Engineering and Environmental and Ecological Engineering at Purdue University.

Major Conclusions & Significance

  • We proposed a biological treatment that uses “cyanophage technology” to control cyanobacteria is a novel and environmentally friendly approach that can be applied to control harmful algal blooms. Hence, the occurrence and accumulation of bloom-forming cyanobacteria in surface waters could be mitigated by cyanophage technology without using chemicals.
  • In essence, we created a biocontrol method that, when applied, should have a low operational cost. To our knowledge, this is the first study to use a cyanophage technique for cyanobacteria remediation in the lake environment.
cyanobacteria bloom model
Figure 2. A simple model showing cyanobacterial blooms with and without cyanophage technology.

What Does This Mean For Indiana?

Our results enhanced the understanding of the relationship between cyanobacteria and cyanophage in Indiana aquatic environments. By understanding and manipulating the relationship, we believe we can create a treatment option that will save money, energy, and time by controlling the occurrence and accumulation of cyanobacteria using the natural cyanophage therapy method. In essence, we are creating a biocontrol method that, when applied, should have a low operational cost. The research outcome will help to increase the quality of freshwaters and hence increase the recreational use and other utilization of the freshwaters in Indiana. This research will benefit students at different levels, engineers, freshwater authorities, and scientists in several different areas. Effective dissemination of the project findings will increase the awareness of the potency of controlling HABs using appropriate cyanophages.

Training The Next Generation

One of the missions of the Indiana Water Resources Research Center, and all Water Centers, is to train the next generation of water scientists. This project successfully funded research for one Masters student within Dr. Simsek’s lab.

 

Contact Laura Esman, Managing Director, to request a printed copy of this factsheet.

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